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KMID : 0604520170430040231
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Journal of the Society of Cosmetic Scientists of Korea
2017 Volume.43 No. 4 p.231 ~ p.237
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Inhibitory Effects of Fractions from Glycine soja Siebold et Zucc. on Melanogenesis in B16F10 Melanoma Cells
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Kim Bo-Ae
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Abstract
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This study was performed to cytotoxicity, tyrosinase inhibition activity, intracellular melanin contents to verify the whitening effect of fraction from Glycine soja Siebold et Zucc. (G. soja). Using western blotting, tyrosinase expression in B16F10 melanoma cells and expression levels of tyrosinase related protein-1 (TRP-1) and protein-2 (TRP-2) were examined. As a result, all of the fractions showed a high cell viability over 82% at the concentrations of 0.125, 0.25, 0.5, 2.0 mg/mL. When the whitening effects of fractions from G. soja were tested using B16F10 melanoma cells treated with the α-melanocyte stimulating hormone (α-MSH), the EtOAc fractions inhibited tyrosinase and melanogenesis effectively. The result of protein expression measurement using western blot showed that TRP-1, TRP-2 and tyrosinase protein expression in B16F10 melanoma cells treated with extracts decreased. Therefore, it is concluded that the fractions from G. soja have whitening effect by inhibiting protein related melanogenesis.
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KEYWORD
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Glycine soja Siebold et Zucc. , melanocyte , tyrosinase , tyrosinase related protein , whitening effect
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